EcoRI and BamHI RFLP polymorphism of Bovine complement c4 gene
نویسندگان
چکیده
منابع مشابه
TaqI RFLP polymorphism of the ovine complement component C4 gene.
Protocol: Reactions were performed as described (2) using 1 ng of DNA and 100 pmoles of each primer. DNA was amplified for 20 cycles using a Perkin Elmer Cetus DNA Thermal Cycler: denaturation, 94°C, 1 min; annealing, 60°C, 1 min; and extension, 70°C, 1.5 min. Ten /il of the reaction was analyzed directly on a 2.5 % NuSieve GTG agarose gel run in Tns-borate buffer. The amplified DNA fragments v...
متن کاملEcoRI polymorphism in the human third complement component (C3) gene.
Source/Description A cDNA fragment, pC3.11 (1) was used as probe. Polymorphisms: EcoRI cleavage of genomic DNA reveals a 2 allele polymorphism with band sizes of 5.2 and 5.6 kb. Invariant bands of 11.5, 11.0, 9.0 and 3 0 kb were also present. Frequency: Estimated from 35 unrelated Caucasians. Allele Frequency Dl 5.2 0.67 D2 5.6 0.33 Chromosomal Localisation: The human C3 gene had been assigned ...
متن کاملHuman gastrin (GAS) RFLP recognised by digestion with BamHI or EcoRI.
SOURCE AUD DESCRIPTION OF CLONE: The human gastrin gene cDNA insert in clone pHG529 (1) is 463 bp in length. This excludes the poly (dC) tail* and includes 8 nucleotides of 5' untranslated region, the entire coding region of 303 nucleotides, a 3 1 untranslated region of 102 nucleotides and a poly (A) track of 50 residues.
متن کاملBamHI RFLP of the inhibin beta B (INHBB) chain gene on chromosome 2.
Source and Description of Clone: pBT A528 contains 920 bp of the inhibin {3B genomic sequence inserted into pBR322 (1). Polymorphism: BamHI identifies two alleles: Allele AI-4.4 kb, Allele A2-3.4 kb.
متن کاملC3 and C4 Complement Levels in Iron Deficiency Anemia
Background and Objectives: Complement proteins are some of the most important plasma proteins of the innate immune system. Impaired immune function is reported in subjects who are iron deficient, and there are documents that these patients are prone to infection. This study was conducted to show whether serum C3 and C4 complement change in adult nonpregnant female with iron deficient anemia or ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1990
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/18.10.3107-a